Similarly, the computed structures of the less active 50a and 50c exhibit an energetic preference towards an upward BCHep conformation, whereas the most active 50b is downwards, consistent with the the solid-state structures (Supplementary Fig
Concurrent medications (metformin, PPIs, antibiotics) and underlying gastrointestinal conditions may cause diarrhoea coinciding with B12 supplementation
1 and Table 2) was performed to confirm that no copies of the GDH alleles or the SAT1 flipper disruption cassette were remaining in C
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(Reconstituted with 3 mL BAC Water)
Western blotting Total protein was extracted from paraformaldehyde-fixed, paraffin-embedded pancreatic tissues using the FFPE Total Protein Extraction Kit (Sangon Biotech, Shanghai, China) or from cultured PSCs using RIPA Lysis Buffer (Beyotime)